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Description
UniOne® TR-FRET Human CRBN Ligand Screening Assay KitProduct Specification Host Human Stability & Storage 80C Background The Human Cereblon (CRBN) Protein Ligand Binding Assay Kit utilizes homogeneous time resolved fluorescence (TR FRET) technology and can be used for the binding detection and screening of CRBN inhibitors or ligands. It enables high throughput screening for rapid detection of CRBN inhibitors or ligands, with the basic principle illustrated in the figure below. The Eu labeled detection
Product Specification
| Host | Human |
| Stability & Storage | -80°C |
Background
The Human Cereblon (CRBN) Protein Ligand Binding Assay Kit utilizes homogeneous time-resolved fluorescence (TR-FRET) technology and can be used for the binding detection and screening of CRBN inhibitors or ligands. It enables high-throughput screening for rapid detection of CRBN inhibitors or ligands, with the basic principle illustrated in the figure below. The Eu-labeled detection reagent (Anti-Tag1-Eu) specifically recognizes and binds to the Tag1-CRBN/DDB1 protein, while the receptor-labeled thalidomide (Thalidomide-Ac) binds to CRBN, bringing Eu and Ac into close proximity. Under excitation by an external light source, energy resonance transfer occurs between the donor and acceptor, and the signal intensity at 665 nm can be measured to determine the binding of CRBN/DDB1 to Thalidomide-Ac. The addition of CRBN ligands or PROTACs to the reaction system competitively binds to CRBN/DDB1, reducing the signal. As the concentration of ligands or PROTACs increases, the signal decreases. This homogeneous assay is simple to perform and does not require washing steps.
Components
Component |
Concentration |
Volume(100T) |
Volume(500T) |
Volume(2500T) |
Volume(10000T) |
Storage Condition |
|---|---|---|---|---|---|---|
Thalidomide |
10 mM in DMSO |
5 μL |
5 μL |
25 μL |
100 μL |
-80℃ |
Tag1-CRBN/DDB1 |
100× |
5μL |
25μL |
125μL |
500 μL |
-80℃ |
Thalidomide-Ac |
100× |
5μL |
25μL |
125μL |
500 μL |
-80℃ |
Anti-Tag1-Eu |
50× |
10μL |
50μL |
250 μL |
1000 μL |
-80℃ |
Detection Buffer |
10× |
250μL |
1.2mL |
6mL |
21 mL |
-80℃ |
*Reagents should be protected from repeated freeze-thaw cycles. It is recommended to aliquot after the first thaw and store according to the storage conditions. The shelf life is one year.
*Thaw reagents on ice before use and equilibrate to room temperature.
*Prepare all reagent working solutions freshly before use.
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy