UniOne® TR-FRET Human Epigeneous Bromodomain Binding Kit
SKU: 85557014907

UniOne® TR-FRET Human Epigeneous Bromodomain Binding Kit

$981.00 $1090.00
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Description

UniOne® TR-FRET Human Epigeneous Bromodomain Binding KitProduct Specification Host Human Stability & Storage 80 Background This kit utilizes Homogeneous Time Resolved Fluorescence(TR FRET) technology to measure the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1 21) peptide. This method enables simple and rapidhigh throughput screening of inhibitors and blocking antibodies. As illustrated, the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1 21) peptide is detected using a Eu labeled Anti

Product Specification


Host Human
Stability & Storage

-80℃

Background

This kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1-21) peptide. This method enables simple and rapidhigh-throughput screening of inhibitors and blocking antibodies.

As illustrated, the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1-21) peptide is detected using a Eu-labeled Anti-Tag1antibody (TR-FRET donor) and an Acceptor-labeled Anti-Tag2 antibody (TR-FRETacceptor). The binding of BRD4 to the [LYS(5,8,12,16)AC] H4(1-21) peptidebrings the donor and acceptor antibodies into close proximity. Excitation ofthe donor antibody then triggers Fluorescence Resonance Energy Transfer (FRET)to the acceptor antibody, resulting in a specific emission signal at 665 nm.The intensity of this specific signal is proportional to the degree ofinteraction between BRD4 and the [LYS(5,8,12,16)AC] H4(1-21) peptide. Thishomogeneous assay is simple to perform and requires no washing steps.



Components

Table 1. Kit Components and Storage Conditions

Component

Concentration

100 TESTS

500 TESTS

2500 TESTS

10000 TESTS

Storage Temp.

(+)-JQ-1

300μM

3μL

10μL

50μL

200μL

-80℃

Tag1-BRD4 protein

100×

4μL

20μL

100μL

400μL

-80℃

Tag2- peptide

100×

4μL

20μL

100μL

400μL

-80℃

Anti-Tag1 Eu dye antibody

50×

10μL

50μL

250μL

1000μL

-80℃

Anti-Tag2 Ac antibody

12.5×

40μL

200μL

1000μL

4000μL

-80℃

Detection buffer

10×

400μL

2mL

10mL

40mL

-80℃

* Avoid repeated freeze-thaw cycles of reagents. Aliquot upon first thaw and store according to the specified conditions. Shelf life is one year.

Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 85557014907

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4.3 ★★★★★
Based on 990 reviews
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B
Verified Purchase
B shops here too much
Port Orchard, US
★★★★★ 2
My raptor GSP destroyed this dino toy
Color: Medium - Dilophosaurus, Color: Medium - Dilophosaurus
My dog ripped this toy apart faster than the trex did the lawyer in Jurassic Park. 2 hours! Arm was ripped off, stuffing pulled out methodically over the next to days. Now it is a gutless husk. The metor was kinder to the og dinos than my gsp was to this. Sorry Bite Force. Open to product sampling tho!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 19, 2026
E
Verified Purchase
Edward Williams
Louisville, US
★★★★★ 4
My Hero pup is super happy with it
Color: Large - Billy the Beast Monster
I was super excited to order this and had waited some time to do so. I wanted Big Billy The Beast Monster in large Green, he’s an awesome looking guy. My Golden Retriever was all over me while I was up packaging Him and his tail was in helicopter mode. He was so excited to have it and carried it around the house everywhere we went, he’s laying on me with his head in my lap and Billy’s right next to him. Reason for buying it? Well he loves plushies but there are not many available out there that can stand up to this 80 pound chew monster so I have to keep him on harder stuff, 2 of which are the cheapest toy skull and crosses in orange and blue from a cheap outlet site but he loves them. Orange was first then when we pulled out the blue one he had a aha moment. Now with Billy he was on a whole new level of excitement, I’ve never seen him this excited over a toy, he can’t stop carrying it with him everywhere and showing it off to the other pups I have, there’s no growling or resource guarding but he definitely won’t want to share and that’s of because I always take away any plushies that he gets to because he is serious about chewing. This being made with Kevlar gave me hopes of longevity but he had the ears and the end of 1 hand chewed off in under 2 hours, am I disappointed…yea a little but he has gotten so much joy out of it that it’s made me really happy to have bought it anyway, see he’s my Service Dog so he spends a lot of time being so serious and working that he needs any outlet for spending energy possible and he’s so happy with it that it makes me happy. As I’m writing this he’s asleep with his head in my lap and Billy right next to him looking very tired and happy and that my friends is worth all the beans in the world.
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Reviewed in the United States on May 4, 2026
J
Verified Purchase
Jodie Lott
Carnegie, US
★★★★★ 5
Survives agressiveness
Color: Large - Billy the Beast Monster
This toy is absolutely for aggressive chewers,my yorkies usually have their stuffed animals torn apart within a day,been a week and not 1 rip! Very happy with it and worth the money!!!
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Reviewed in the United States on May 29, 2026
J
Verified Purchase
John
West Palm Beach, US
★★★★★ 4
Great except for one small flaw
Color: Regular - Beak, Color: Regular - Beak
Our dog likes to get at the juicy innards of every toy we buy him. We tried this one as it promised to be "bullet proof". It lasted about a week which in fairness is 6.5 days longer than most of the toys we buy last. He ripped off the wings which was to be expected and not an issue. It took him another 6 days to finally locate a weak point and break in. It appears that the tag is not made of kevlar but merely sewed on. I guess it would be better if they had just sewed it on top but they made it part of the overall structural integrity. This provided a small area of weakness which our dog located and proceeded to exploit. It's like having the death star but installing a small thermal exhaust port that allows for access to the very core of the station. Makes little sense. Overall I am pleased with the overall sturdiness but in the end this toy had a small design flaw that my dog was able to locate and exploit in his neverending quest to gain access to and eat polyester fiber stuffing
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 6, 2026
K
Verified Purchase
Kym Brunner
Dallas, US
★★★★★ 5
STRONG AS A MONSTER (albeit a cute one)
Color: Large - Billy the Beast Monster, Color: Large - Billy the Beast Monster
My aggressive chewer ate the adorable monster's ear off on the first day, but who cares? The body of the monster has been indestructible so far (one week). My 7 month old pup cbews it for a good 10-15 minutes at a time several times per day. Great quality product and outlasting all the other toys I bought (which she destroyed in 10 minutes - even the ones rated "Level 4 toughness" from Walmart. I think it's hilarious in my photo that the monster's expression perfectly shows his disappointment about missing an ear, LOL.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 3, 2026

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