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Description
Human SALL1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Sal-like protein 1 (SALL1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Sal-like protein 1 (SALL1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Sal-like protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Sal-like protein 1 (SALL1) is a protein encoded by the SALL1 gene. The protein encoded by this gene is a zinc finger transcriptional repressor and may be part of the NuRD histone deacetylase (HDAC) complex. Defects in this gene contribute to Townes-Brocks syndrome (TBS) and Branchio-oto-renal syndrome (BOR). Two transcript variants encoding different isoforms of this gene have been identified. SALL1 has been shown to interact with TERF1 and UBE2I. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
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Product Reviews
★★★★★ 1
Not durable
Color: Nude, Size: Women 9-13
Only used two of them so far. One sock lasted nearly 3 wears. The other broke before I could finish wearing it the second time. Basically useless junk.
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Reviewed in the United States on April 2, 2026
★★★★★ 4
Thick Silicone Moisturizing Socks
Color: Nude, Size: Women 9-13, Color: Nude, Size: Women 9-13
★★★★☆ Thick Silicone Moisturizing Socks
I ordered these silicone moisturizing socks to try for dry heels. Unlike typical fabric spa socks, these are made entirely of soft silicone and are noticeably thicker and very stretchy.
When I first took them out of the package there was a visible fold line in the silicone. It appears to be from how they were folded in the packaging rather than a defect in the material. The silicone itself feels durable and flexible.
The design works like a full silicone sleeve that traps moisturizer against your skin. I applied Aquaphor first and then slid these on so the moisture stays in contact with the skin longer. One thing I noticed right away is that the silicone feels cool when you first put them on, which is actually pretty comfortable.
The bottoms have a zig-zag textured pattern that gives some grip if you need to stand briefly, though these seem better for relaxing while moisturizer absorbs rather than walking around much.
The ankle opening is the tightest part of the sock. It stretches, but it’s noticeably snug compared to the rest of the silicone, which helps keep them from sliding off.
For sizing reference, I wear a women’s 7.5–8 shoe and the size Large fits comfortably. There’s plenty of stretch and I can wiggle my toes easily, so they should accommodate larger feet as well.
They’re also easy to clean. After using them you can simply wash them with soap and water and let them dry completely before storing or using them again.
Overall these appear well made with thick silicone and plenty of stretch. If you use moisturizing socks or heel treatments, these work well for keeping lotion in place while your feet absorb it.
Since this set comes with two pairs for about $10, the price seems pretty reasonable for something reusable like this.
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Reviewed in the United States on March 6, 2026
★★★★★ 5
Shimmer, Shine, and All the Glitz ✨💖
Color: Diamond White
I am a girl who loves to shimmer, shine, glitz, and glow, so this Boko Highlighter Powder Spray was an absolute delight to try! It adds a gorgeous sparkling finish that really bedazzles my skin, making me feel extra glamorous whether I’m going out or just playing with my makeup.
The spray is easy to apply and gives a smooth, even shimmer without feeling gritty or heavy. I’ve been using it for daily wear, as well as for little festival vibes and fun nights out, and it really catches the light beautifully.
If you love sparkle and want to elevate your glow game, this is such a fun, high-quality product to add to your collection! 🌟💎✨
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Reviewed in the United States on March 12, 2026
★★★★★ 4
Classic old-fashioned presentation, versatile uses
Color: Diamond White, Color: Diamond White
I loved the presentation of this glitter in an old-fashioned atomizer perfume bottle with a pink squeeze puff. Because glitter powder is not prone to evaporation, an atomizer is actually a great way to package powder.
I tried this product with the atomizer and without. Honestly, I was just looking for some sparkles on my cheekbones and a glitter streak in my hair for a New Years Party. The instructions advised that for a targeted glitter application, spray the glitter on your hand and apply it with a brush. I found that this way left too much glitter behind on my hand.
Instead, I shook some glitter onto a piece of paper and mixed some with Aquaphor for application on my face. This helped the glitter adhere to my skin and lasted for hours. I mixed some glitter into hair gel and applied it to a streak in my hair. The larger pieces of silver glitter really sparkled this way.
Overall, this packaging looks great and would be functional if you were looking for an all-over application. Otherwise, just using a simple bottle works as well.
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Reviewed in the United States on December 31, 2025
★★★★★ 5
Woah, Shimmer!
Color: Late Autumn, Color: Late Autumn
I love cute little things like this with the spray bulb. They’re fun gifts. My daughter likes to shimmer sometimes. She kind of overdid it the first time. It does t take much to get a sparkly, fine shimmer. The photos are a bit exaggerated on the product page. You don’t have to “paint” your skin with it. It’s an easy application, and when used right really provides a nice accent. It’s light and has a quality look and feel.
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Reviewed in the United States on January 10, 2026