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Ships within 48 hours · Estimated delivery Jul 14 - Jul 19
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Description
Human H2AFX (Histone H2AX) ELISA KitThe Human H2AFX (Histone H2AX) ELISA Kit measures H2AFX in Human Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti H2AFX antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with H2AFX bound to
The Human H2AFX (Histone H2AX) ELISA Kit measures H2AFX in Human Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti H2AFX antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with H2AFX bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of H2AFX in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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Exchange/Return Notes
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