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Description
bsa fatty acid free cho vero mdckPremium fr CHO, Vero, MDCK & sensitive Assays SeamlessBio Bovine Serum Albumin Fatty Acid Free ist speziell aufgereinigt, um gebundene Fettsuren zu entfernen, die in Standard BSA natrlich vorkommen. Mit 98% Reinheit und <5 EU mg Endotoxin ist es die erste Wahl fr Fettsure sensitive Zelllinien (CHO, Vero, MDCK) und Lipid Metabolismus Studien. Fatty Acid Free >99% FA Entfernung durch Charcoal Delipidation Reinheit 98% Hher als Standard BSA Low Endotoxin
Premium für CHO, Vero, MDCK & sensitive Assays
SeamlessBio Bovine Serum Albumin Fatty Acid Free ist speziell aufgereinigt, um gebundene Fettsäuren zu entfernen, die in Standard-BSA natürlich vorkommen. Mit ≥98% Reinheit und <5 EU/mg Endotoxin ist es die erste Wahl für Fettsäure-sensitive Zelllinien (CHO, Vero, MDCK) und Lipid-Metabolismus-Studien.
✓ Fatty Acid Free – >99% FA-Entfernung durch Charcoal-Delipidation
✓ Reinheit ≥98% – Höher als Standard-BSA
✓ Low Endotoxin <5 EU/mg – LAL-tested
✓ Keine Interferenz – Ideal für Lipid-sensitive Assays
✓ AU/NZ/US Origin – BSE/TSE-frei
✓ Reinheit ≥98% – Höher als Standard-BSA
✓ Low Endotoxin <5 EU/mg – LAL-tested
✓ Keine Interferenz – Ideal für Lipid-sensitive Assays
✓ AU/NZ/US Origin – BSE/TSE-frei
Warum Fatty Acid Free BSA?
Standard-BSA enthält natürlich gebundene Fettsäuren (~0.5-2 mol FA pro mol BSA), hauptsächlich:
- Palmitate (C16:0) – Gesättigte Fettsäure
- Stearate (C18:0) – Gesättigte Fettsäure
- Oleate (C18:1) – Einfach ungesättigte Fettsäure
- Linoleate (C18:2) – Mehrfach ungesättigte Fettsäure
Probleme in bestimmten Anwendungen:
- Zellkultur: CHO, Vero, MDCK sind sensitiv gegenüber exogenen Fettsäuren → veränderte Wachstumsraten, beeinträchtigte Produktqualität
- Lipid-Metabolismus-Studien: Native FA interferieren mit Messungen von Lipid-Transportern, Beta-Oxidation, Lipoprotein-Metabolismus
- Hormon-Assays: FA beeinflussen Steroid-Bindung (Testosteron, Estrogen, Cortisol)
- Drug-Binding-Studies: FA konkurrieren um Bindungsstellen am Albumin
Lösung: Fatty Acid Free BSA eliminiert diese Probleme durch Charcoal-Delipidation (Aktivkohle-Behandlung) → >99% FA-Entfernung.
Technische Spezifikationen
| Parameter | Spezifikation | Test-Methode |
|---|---|---|
| Quelle | Rinderplasma (AU/NZ/US) | COO-Zertifikat |
| Reinheit | ≥98% | Elektrophorese |
| Fettsäure-Gehalt | <0.01 mol FA/mol BSA | GC-MS (>99% Entfernung) |
| Endotoxin | <5 EU/mg | LAL-Test |
| Albumin-Gehalt | ≥98 g/L | Bradford/BCA |
| Monomere | ≥95% | SEC-HPLC |
| pH-Wert | 6.8-7.5 | pH-Meter |
| Format | Lyophilized Powder | — |
| Lagerung | Raumtemperatur (versiegelt) | — |
| Haltbarkeit | 3 Jahre | — |
Standard vs. Fatty Acid Free BSA
| Eigenschaft | Standard BSA | Fatty Acid Free BSA |
|---|---|---|
| Fettsäure-Gehalt | 0.5-2 mol FA/mol BSA | <0.01 mol FA/mol BSA |
| Reinheit | ≥96% | ≥98% |
| Endotoxin | <10 EU/mg | <5 EU/mg |
| Ideal für | Western Blot, ELISA, Protein-Standard | CHO/Vero/MDCK, Lipid-Studien |
Anwendungen
1. CHO/Vero/MDCK Zellkultur
Warum diese Zelllinien Fatty Acid Free BSA brauchen:
CHO (Chinese Hamster Ovary):
- Biomanufacturing von monoklonalen Antikörpern (mAbs) und rekombinanten Proteinen
- Exogene Fettsäuren stören Glykosylierung und Produktqualität
- FDA/EMA bevorzugen FA-freie Supplements für GMP-Produktion
- Typische Konzentration: 0.5-2 g/L in serum-freien Medien
Vero (African Green Monkey Kidney):
- Vaccine Production (Polio, Rabies, Rotavirus, Dengue)
- WHO-zertifizierte Zelllinie für humane Impfstoffe
- FA-freie Bedingungen sind essentiell für regulatorische Compliance
- Verbesserte Virus-Titer bei FA-freiem BSA
MDCK (Madin-Darby Canine Kidney):
- Influenza-Vaccine Production (saisonale und pandemische Impfstoffe)
- FDA bevorzugt FA-freie Supplements
- Höhere HA (Hemagglutinin) Ausbeute
2. Lipid-Metabolismus & Fatty Acid Uptake Studies
Studien zu Lipid-Transportern:
- CD36 (Fatty Acid Translocase): Uptake-Assays ohne Background durch native FA
- FATP (Fatty Acid Transport Protein): Spezifische FA-Aufnahme-Studien
- FABPpm (Plasma Membrane Fatty Acid Binding Protein): Bindungs-Studien
Beta-Oxidation-Assays:
- Messung der mitochondrialen FA-Oxidation
- Keine Interferenz durch BSA-gebundene FA
- Verwendung radioaktiv-markierter FA (z.B. 14C-Palmitat)
Lipoprotein-Metabolismus:
- HDL/LDL-Studien
- Apolipoprotein-Bindung
- CETP (Cholesteryl Ester Transfer Protein) Aktivität
Adipozyten-Differenzierung:
- 3T3-L1 Präadipozyten → Adipozyten
- Kontrollierte FA-Zugabe für Lipid-Akkumulation
- Oil Red O Färbung ohne Background
3. Hormone-Binding Studies
Thyroid Hormone (T3, T4) Binding:
- FA konkurrieren um Bindungsstellen am Albumin
- Fatty Acid Free BSA ermöglicht präzise Bindungsstudien
- Wichtig für Schilddrüsen-Diagnostik
Steroid Hormone Assays:
- Estrogen, Testosteron, Cortisol, Progesteron
- FA beeinflussen Protein-Bindung
- Wichtig für Endokrinologie-Forschung
Drug-Protein Binding Studies:
- Pharmakokinetik-Studien
- Kompetition zwischen Drug und FA um Albumin-Bindungsstellen
- Wichtig für Drug Development
4. Serum-freie Medien Formulierung
- Präzise Kontrolle über Lipid-Zusammensetzung
- Gezielte Zugabe definierter Fettsäuren (z.B. Oleic Acid, Linoleic Acid)
- Reproduzierbare Zellkultur-Bedingungen
- GMP-konforme Medien-Entwicklung
Verfügbare Volumina
| Format | Packaging | Ideal für |
|---|---|---|
| Lyophilized Powder | 10 g | Kleine Labs, Methodenentwicklung |
| Lyophilized Powder | 50 g | Routine-Forschung |
| Lyophilized Powder | 100 g | High-throughput Labs |
| Lyophilized Powder | 500 g | Bioproduktion, GMP |
Andere Volumina & Bulk-Orders: Größere Mengen (≥1 kg) sowie kundenspezifische Formate sind auf Anfrage verfügbar. Kontaktieren Sie uns für ein individuelles Angebot.
Lagerung & Handling
Lagerung:
- Raumtemperatur (15-25°C), trocken, versiegelt
- Haltbarkeit: 3 Jahre ab Herstellung
- Nach Öffnen: Bei 2-8°C lagern, innerhalb 6 Monaten verbrauchen
Auflösen von Pulver:
- Langsam in Wasser oder Puffer (PBS, TBS) geben
- Vorsichtig schwenken (nicht vortexen)
- Bei Raumtemperatur oder 37°C lösen
- pH auf 7.0-7.4 einstellen
- 0.2 µm sterilfiltrieren für Zellkultur
Wichtig: Vermeiden Sie wiederholtes Einfrieren/Auftauen, da dies zu Proteinaggregation führen kann.
Herkunft & Qualitätssicherung
AU/NZ/US – BSE/TSE-freie Regionen (OIE-zertifiziert)
Jede Charge wird geliefert mit:
- Certificate of Analysis (CoA) – Vollständige Spezifikationen
- Certificate of Origin (COO) – AU/NZ/US Herkunft dokumentiert
- Fatty Acid Analysis (GC-MS) – <0.01 mol FA/mol BSA bestätigt
- Endotoxin-Test-Zertifikat (LAL) – <5 EU/mg bestätigt
- BSE/TSE-Freiheitserklärung
- Safety Data Sheet (SDS)
Warum SeamlessBio für BSA Fatty Acid Free?
- >99% FA-Entfernung – Charcoal-Delipidation validiert
- Reinheit ≥98% – Höher als Standard-BSA
- Low Endotoxin <5 EU/mg – Ideal für Zellkultur
- BSE/TSE-frei – AU/NZ/US Origin
- Schnelle EU-Lieferung – Lager in Deutschland, 1-3 Tage
- Kostenfreie Muster – 10g Test-Volume für qualifizierte Kunden
- Technischer Support – Bei Fragen zu Protokollen
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.3 ★★★★★
Based on 1128 reviews
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Product Reviews
★★★★★ 5
Quickly accepted by our local hummingbirds.
Size: 64 Fl Oz (Pack of 1), Style: 64 oz Ready To Use
I used this in a red hanging hummingbird feeder on a second-floor shaded balcony. The feeder had only been up a short time, and the nectar was accepted quickly. I observed hummingbirds visiting, perching, and drinking from the active lower tiers, so the product clearly did not discourage feeding.
What I like most is the convenience. It is ready to pour, clear, and does not require mixing sugar and water. I also appreciate that it is not dyed red. The feeder itself provides enough red visual attraction, so colored liquid is not necessary. For a small balcony feeder, I only needed to fill the reservoirs about halfway, which helped keep the nectar fresh and reduced waste.
A few practical notes: I still treat this like any hummingbird nectar and replace it regularly rather than letting it sit for many days. Even in shaded, mild weather, I would rather keep the feeder clean and fresh. The bottle should also be stored properly after opening.
Overall, this is a good option if you want a simple ready-to-use nectar without mixing your own. Homemade sugar water is cheaper, but this is convenient, clean-looking, and in my case the hummingbirds used it. I would recommend it for someone who wants an easy pour-and-fill option, especially for a smaller feeder or balcony setup.
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Hummingbirds love it… ants do too 🐦🐜
Size: 64 Fl Oz (Pack of 1), Style: 64 oz Ready To Use
This nectar definitely does what it’s supposed to do — the hummingbirds found it fast and keep coming back, which is exactly what I wanted. It’s easy to use and convenient compared to mixing your own.
The only downside is that ants seem to love it just as much as the birds. If you’re using this, I’d definitely recommend pairing it with an ant moat or being mindful of where you hang your feeder.
Overall, it works great and brings in plenty of hummingbirds — just be prepared to outsmart a few tiny freeloaders along the way.
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Reviewed in the United States on April 29, 2026
★★★★★ 5
Loved by the birds
Size: 64 Fl Oz (Pack of 1), Style: 64 oz Ready To Use
Easy to pour and the birds love it. I keep ordering this per the hummingbird's instructions!
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Reviewed in the United States on May 12, 2026
★★★★★ 5
Soft, Luxurious & Perfect for Hair and Skin!
Size: Queen (20" X 30"), Color: 11 - Light Turquoise, Number of Items: 2, Size: Queen (20" X 30"), Color: 11 - Light Turquoise, Number of Items: 2
These satin pillowcases are honestly amazing. The material feels super soft, smooth, and cooling on the skin, and they make my bed look so elegant and luxurious. I bought them mainly to help protect my hair from frizz and dryness, They’re also very comfortable to sleep on and don’t feel cheap at all. The color is beautiful in person, exactly like the photos on Amazon. I washed them already and they still kept their softness and shine. Definitely worth the purchase for the price I would 100% buy again and recommend to anyone looking for cute, comfortable satin pillowcases.”
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Budget friendly satin pillowcase
Size: Standard (20" X 26"), Color: 18 - Lavender, Number of Items: 2
This satin pillowcase is a great budget-friendly option! While it’s not quite as luxurious as my Blissy pillowcase, it still does a really nice job—especially for the price.
It has that smooth, silky feel that helps reduce hair frizz and feels gentle on the skin. It’s also easy to wash and holds up well, which is perfect for everyday use. I especially love using these for the kids—they get the benefits of a softer pillowcase without worrying about ruining a more expensive one.
Overall, it’s a solid, affordable alternative that still feels nice and does the job. Great value and perfect for families!
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Reviewed in the United States on April 22, 2026
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